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We present a new method to infer the 3D luminosity distributions of edge-on barred galaxies with boxy-peanut/X (BP/X) shaped structures from their 2D surface brightness distributions. Our method relies on forward modelling of newly introduced parametric 3D density distributions for the BP/X bar, disc and other components using an existing image fitting software package (imfit). We validate our method using an N- body simulation of a barred disc galaxy with a moderately strong BP/X shape. For fixed orientation angles, the derived 3D BP/X-shaped density distribution is shown to yield a gravitational potential that is accurate to at least 5 per cent and forces that are accurate to at least 15 per cent, with average errors being |$\sim 1.5~{{\ \rm per\ cent}}$| for both. When additional quantities of interest, such as the orientation of the bar to the line of sight, its pattern speed, and the stellar mass-to-light ratio are unknown they can be recovered to high accuracy by providing the parametric density distribution to the Schwarzschild modelling code FORSTAND. We also explore the ability of our models to recover the mass of the central supermassive black hole. This method is the first to be able to accurately recover both the orientation of the bar to the line of sight and its pattern speed when the disc is perfectly edge-on. [ABSTRACT FROM AUTHOR]

Background: Foliar diseases namely late leaf spot (LLS) and leaf rust (LR) reduce yield and deteriorate fodder quality in groundnut. Also the high oleic acid content has emerged as one of the most important traits for industries and consumers due to its increased shelf life and health benefits. Results: Genetic mapping combined with pooled sequencing approaches identified candidate resistance genes (LLSR1 and LLSR2 for LLS and LR1 for LR) for both foliar fungal diseases. The LLS-A02 locus housed LLSR1 gene for LLS resistance, while, LLS-A03 housed LLSR2 and LR1 genes for LLS and LR resistance, respectively. A total of 49 KASPs markers were developed from the genomic regions of important disease resistance genes, such as NBS-LRR, purple acid phosphatase, pentatricopeptide repeat-containing protein, and serine/threonine-protein phosphatase. Among the 49 KASP markers, 41 KASPs were validated successfully on a validation panel of contrasting germplasm and breeding lines. Of the 41 validated KASPs, 39 KASPs were designed for rust and LLS resistance, while two KASPs were developed using fatty acid desaturase (FAD) genes to control high oleic acid levels. These validated KASP markers have been extensively used by various groundnut breeding programs across the world which led to development of thousands of advanced breeding lines and few of them also released for commercial cultivation. Conclusion: In this study, high-throughput and cost-effective KASP assays were developed, validated and successfully deployed to improve the resistance against foliar fungal diseases and oleic acid in groundnut. So far deployment of allele-specific and KASP diagnostic markers facilitated development and release of two rust- and LLS-resistant varieties and five high-oleic acid groundnut varieties in India. These validated markers provide opportunities for routine deployment in groundnut breeding programs. [ABSTRACT FROM AUTHOR]

Microorganisms produce siderophores, which are low-molecular-weight iron chelators when iron availability is limited. The present analyzed the role of LNPF1 as multifarious PGPR for improving growth parameters and nutrient content in peanut and soil nutrients. Such multifarious PGPR strains can be used as effective bioinoculants for peanut farming. In this work, rhizosphere bacteria from Zea mays and Arachis hypogaea plants in the Salem area of Tamil Nadu, India, were isolated and tested for biochemical attributes and characteristics that stimulate plant growth, such as the production of hydrogen cyanide, ammonia (6 µg/mL), indole acetic acid (76.35 µg/mL), and solubilizing phosphate (520 µg/mL). The 16S rRNA gene sequences identified the isolate LNPF1 as Pseudomonas fluorescens with a similarity percentage of 99% with Pseudomonas sp. Isolate LNPF1 was evaluated for the production of siderophore. Siderophore-rich supernatant using a Sep Pack C18 column and Amberlite-400 Resin Column (λmax 264) produced 298 mg/L and 50 mg/L of siderophore, respectively. The characterization of purified siderophore by TLC, HPLC, FTIR, and 2D-NMR analysis identified the compound as desferrioxamine, a hydroxamate siderophore. A pot culture experiment determined the potential of LNPF1 to improve iron and oil content and photosynthetic pigments in Arachis hypogaea L. and improve soil nutrient content. Inoculation of A. hypogea seeds with LNPF1 improved plant growth parameters such as leaf length (60%), shoot length (22%), root length (54.68%), fresh weight (47.28%), dry weight (37%), and number of nuts (66.66) compared to the control (untreated seeds). This inoculation also improved leaf iron content (43.42), short iron content (38.38%), seed iron (46.72%), seed oil (31.68%), carotenoid (64.40%), and total chlorophyll content (98.%) compared to control (untreated seeds). Bacterized seeds showed a substantial increase in nodulation (61.65%) and weight of individual nodules (95.97) vis-à-vis control. The results of the present study indicated that P. fluorescens might be utilized as a potential bioinoculant to improve growth, iron content, oil content, number of nuts and nodules of Arachishypogaea L., and enrich soil nutrients. [ABSTRACT FROM AUTHOR]

An attempt was made to study the response of nano-sulfur in groundnut using four levels of nano-sulfur (NS) and conventional sulfur (CS) fertilizers in a completely randomized block design and replicated thrice. The results indicated that application of nano-sulfur @ 30 kg S ha−1 recorded 0.76 mg root sulfur uptake plant−1, 40.5 mg shoot sulfur uptake plant−1, 14.9 mg kernel sulfur uptake plant−1, and 3.09 mg shell sulfur uptake plant−1 higher in comparison to conventional sulfur @ 40 kg S ha−1 recorded root, shoot, kernel and shell sulfur uptake of 0.53, 35.8, 11.4 and 2.46 mg plant−1, respectively. The highest pod yield was recorded at 12.4 g plant−1 with nano-sulfur application @ 30 kg S ha−1 when compared to conventional sulfur @ 40 kg S ha−1 registered at 10.7 g plant−1. Quality parameters such as oil, crude protein, methionine, cysteine, and total free amino acid content were recorded highest under nano-sulfur application @ 30 kg S ha−1 i.e. 48.3%, 27.2%, 3.44 mg g −1, 1.89 mg g−1 and 46.3 mg g−1 respectively whereas conventional sulfur @ same set of sulfur treatment observed (46.7% oil, 25.1% crude protein, 3.16 mg methionine g−1, 1.75 mg cysteine g−1, and 39.6 mg total free amino acids g−1. Finally, the results concluded that the application of nano-S @ 30 kg S ha−1 is sufficient to attain higher sulfur use efficiency with a reduction of sulfur fertilizer to the tune of 25% besides augmenting the soil sulfur reserve without harming the environment. [ABSTRACT FROM AUTHOR]

Background: This study aims to decipher the genetic basis governing yield components and quality attributes of peanuts, a critical aspect for advancing molecular breeding techniques. Integrating genotype re-sequencing and phenotypic evaluations of seven yield components and two grain quality traits across four distinct environments allowed for the execution of a genome-wide association study (GWAS). Results: The nine phenotypic traits were all continuous and followed a normal distribution. The broad heritability ranged from 88.09 to 98.08%, and the genotype-environment interaction effects were all significant. There was a highly significant negative correlation between protein content (PC) and oil content (OC). The 10× genome re-sequencing of 199 peanut accessions yielded a total of 631,988 high-quality single nucleotide polymorphisms (SNPs), with 374 significant SNP loci identified in association with the nine traits of interest. Notably, 66 of these pertinent SNPs were detected in multiple environments, and 48 of them were linked to multiple traits of interest. Five loci situated on chromosome 16 (Chr16) exhibited pleiotropic effects on yield traits, accounting for 17.64–32.61% of the observed phenotypic variation. Two loci on Chr08 were found to be strongly associated with protein and oil contents, accounting for 12.86% and 14.06% of their respective phenotypic variations, respectively. Linkage disequilibrium (LD) block analysis of these seven loci unraveled five nonsynonymous variants, leading to the identification of one yield-related candidate gene and two quality-related candidate genes. The correlation between phenotypic variation and SNP loci in these candidate genes was validated by Kompetitive allele-specific PCR (KASP) marker analysis. Conclusions: Overall, molecular markers were developed for genetic loci associated with yield and quality traits through a GWAS investigation of 199 peanut accessions across four distinct environments. These molecular tools can aid in the development of desirable peanut germplasm with an equilibrium of yield and quality through marker-assisted breeding. [ABSTRACT FROM AUTHOR]

In many parts of the world, including Cameroon, mycotoxin contamination of groundnuts remains a major constraint affecting their use as food. Understanding the contributing factors is an essential intervention to reduce contamination and people's exposure to these harmful toxins. The aim of this research was to identify the factors associated with the knowledge, perceptions, and postharvest practices of groundnut farmers in three production basins in Cameroon. Data were collected through surveys and analyzed using descriptive methods and logistic regression models. The results show that groundnut farmers are little aware of the existence of mycotoxins (12%) and totally unaware of the existence of aflatoxins (100%). Only 7.1% of these farmers are aware of the effects of mycotoxins on consumer health. After evaluation, the large majority of farmers scored poor marks for knowledge (86%) and practice (98.7%) in the management of mould and mycotoxins in groundnuts. Knowledge of mycotoxins was positively associated with the level of education [OR = 3.42; (95%-IC: 1.00–16.00); p < 0.05 ] and region [OR = 4.49; (95%-IC: 1.09–20.3); p < 0.05 ]. Farmers' good practices were linked to their production experience [OR = 6.06, (95% CI: 0.91-18.4), p = 0.035 ]. The use of mouldy groundnut for feed was associated with age [OR = 3.34, (95% CI: 1.14–10.2), p = 0.03 ], sex [OR = 0. 43, (IC-95%: 1.14–1.05), p = 0.026 ], marital status [OR = 0.35, (IC-95%: 0.14–0.79), p = 0.015 ], and production region [OR = 0.27, (IC-95%: 0.13–0.56)]. In conclusion, groundnut farmers had insufficient knowledge of mycotoxins, no knowledge of aflatoxins, and suboptimal handling and storage practices for this commodity. This contributes to increasing the risk of exposure for the population and requires mitigation measures, including awareness campaigns on mycotoxins, specifically aflatoxins, and capacity building for farmers in terms of storage and postharvest management of foodstuffs. [ABSTRACT FROM AUTHOR]

In peanut allergy, Arachis hypogaea 2 (Ara h 2) and Arachis hypogaea 6 (Ara h 6) are two clinically relevant peanut allergens with known structural and sequence homology and demonstrated cross-reactivity. We have previously utilized X-ray crystallography and epitope binning to define the epitopes on Ara h 2. We aimed to quantitatively characterize the cross-reactivity between Ara h 2 and Ara h 6 on a molecular level using human monoclonal antibodies (mAbs) and structural characterization of allergenic epitopes. We utilized mAbs cloned from Ara h 2 positive single B cells isolated from peanut-allergic, oral immunotherapy–treated patients to quantitatively analyze cross-reactivity between recombinant Ara h 2 (rAra h 2) and Ara h 6 (rAra h 6) proteins using biolayer interferometry and indirect inhibitory ELISA. Molecular dynamics simulations assessed time-dependent motions and interactions in the antibody–antigen complexes. Three epitopes—conformational epitopes 1.1 and 3, and the sequential epitope KRELRNL/KRELMNL—are conserved between Ara h 2 and Ara h 6, while two more conformational and three sequential epitopes are not. Overall, mAb affinity was significantly lower to rAra h 6 than it was to rAra h 2. This difference in affinity was primarily due to increased dissociation of the antibodies from rAra h 6, a phenomenon explained by the higher conformational flexibility of the Ara h 6–antibody complexes in comparison to Ara h 2–antibody complexes. Our results further elucidate the cross-reactivity of peanut 2S albumins on a molecular level and support the clinical immunodominance of Ara h 2. We utilized monoclonal antibodies cloned from peanut-allergic, oral immunotherapy–treated patients to probe the cross-reactivity between clinically relevant peanut allergens Ara h 2 and Ara h 6 on a molecular level. Graphical Abstract [ABSTRACT FROM AUTHOR]

BACKGROUND: Biopolymer‐based microgels are being regarded increasingly as promising building blocks in food applications. This study aimed to clarify the evolution process of the network for fat analogue emulsions stabilized by peanut protein isolate (PPI) microgel particles. It also investigated the interfacial structure and characteristics of emulsions (50% oil phase, w/w) stabilized by microgels under different pH conditions. RESULTS: There was an increasing interfacial adsorption capacity for PPI microgels over time (from 85.26% to the maximum of 89.78% at 24 h of storage) due to the aggregation of microgels around droplets and the development of cross‐linking microgel chains between adjacent interfaces. The increased β‐sheet content (from 35.51% to 41.12%) of adsorbed microgels indicated unfolding and the enhanced aggregation of microgels, which led to stronger droplet interaction. The network evolution observed with different microscopes clarified the transition to a self‐supporting emulsion. The uneven adsorption of large microgel aggregates at the oil–water interface promoted larger and deformed droplets, so more fat‐like medium internal phase emulsion stabilized by PPI microgel could be obtained by adjusting the microgel pH to 4.5. The interfacial membranes observed by scanning electron microscopy were thicker and coarser at pH 3.0 and 4.5 than those at pH 7.0 and 9.0. The adsorption of PPI microgel aggregates enhanced the structural strength and improved emulsion stability. CONCLUSION: This work could form a basis for further studies relating physical properties to the design of plant protein‐based fat analogues. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Summary: Peanuts produce millions of tons of high‐protein peanut meal (after oil extraction) annually that can be used for food. Peanut protein, as a plant‐based protein, is a great alternative to animal protein to address protein deficiencies and rising health problems in the world. It has a high nutritional value, while the globular structure results in poor functional properties, limiting its application. Better functional properties can be obtained through processing and modification. This paper elaborates the nutritional and functional properties of peanut protein and highlights the application of peanut protein in food industry in recent years. Applications for peanut protein include meat and milk substitutes, edible films, nanoparticles, and some new technologies such as electrostatic spinning and three‐dimensional (3D) printing in recent years. This paper could improve people's understanding of the molecular structure of peanut protein and the physicochemical properties associated with the structure changes during processing. It could also contribute to better utilisation of peanut protein, providing more varieties of peanut protein products for the food industry. [ABSTRACT FROM AUTHOR]

Summary: This study aimed to enhance the functional properties of peanut protein hydrolysates (PPHs) by coupling limited enzymatic hydrolysis with chlorogenic acid (CGA) modification, thus offering novel insights into the potential food industry applications of peanut proteins. Initially, peanut protein isolate was hydrolysed using trypsin. Subsequently, covalent conjugation with CGA was achieved via free‐radical grafting. The moderately hydrolysed 4% PPHs had the highest polyphenol‐binding capacity. Measurement of the PPHs‐CGA complex revealed that free amino, thiol, and tryptophan contents were reduced. The amino acid content results also showed that cysteine, lysine, arginine, and methionine (with amino or thiol groups) were the primary binding sites. Spherical or rod‐like structures were observed in the scanning electron microscopy images of PPHs‐CGA, and its particle size was larger than that of the PPHs. After combining PPHs and CGA, the fluorescence intensity decreased, Fourier‐transform infrared spectrum showed a shift in the absorption peak, and electrophoresis band weakened, indicating that covalent binding was successful. PPHs with different degrees of hydrolysis have different levels of exposure to amino acids, and their antioxidant properties differ when combined with CGA. After combining, the surface hydrophobicity was reduced, and the secondary structure was unfolded and extended, thereby improving its emulsifying properties. In summary, PPHs‐CGA may be used in the food industry as a bi‐type food factor and proposes a new application for peanut protein in food. [ABSTRACT FROM AUTHOR]

Summary: N6‐methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes and is an important regulator of gene expression as well as many other critical biological processes. However, the characteristics and functions of m6A in peanut (Arachis hypogea L.) resistance to bacterial wilt (BW) remain unknown.Here, we analyzed the dynamic of m6A during infection of resistant (H108) and susceptible (H107) peanut accessions with Ralstonia solanacearum (R. solanacearum), the causative agent of BW. Throughout the transcriptome, we identified 'URUAY' as a highly conserved motif for m6A in peanut. The majority of differential m6A located within the 3′ untranslated region (UTR) of the transcript, with fewer in the exons.Integrative analysis of RNA‐Seq and m6A methylomes suggests the correlation between m6A and gene expression in peanut R. solanacearum infection, and functional analysis reveals that m6A‐associated genes were related to plant‐pathogen interaction.Our experimental analysis suggests that AhALKBH15 is an m6A demethylase in peanut, leading to decreased m6A levels and upregulation of the resistance gene AhCQ2G6Y. The upregulation of AhCQ2G6Y expression appears to promote BW resistance in the H108 accession. [ABSTRACT FROM AUTHOR]

The application of zinc (Zn) and calcium (Ca) fertilizers is necessary to improve quantitative and qualitative yield of groundnut. This study was done to evaluate seed yield and the fatty acids composition of groundnut (Arachis hypogaea L.) in response to the different forms of Zn- and Ca-based fertilizers. This experiment is carried out as a split plot arrangement based on randomized complete block design with three replications in Rasht, Iran, during the 2018 and 2019 cropping seasons. The four levels of Zn fertilizers including without fertilizer application (control), Zn Nano-fertilizer (Zn-Nano), Zn-EDTA, and Zn-sulfate (ZnSO4) as the main plot and the four levels of the Ca fertilizers including Ca Nano-fertilizer (Ca-Nano), Ca-EDTA, Ca-chloride (CaCl2), and Ca-nitrate [Ca(NO3)2] as the sub-plot comprised experimental treatments. The greatest pod number per plant (PNP) (38.78), 100-seed weight (100-SW) (61.49 g), seed yield (SY) (2790 kg/ha), biological yield (BY) (9303 kg/ha), seed oil content (SOC) (52.34%), oil yield (OY) (1625 kg/ha), protein yield (PY) (673 kg/ha), oleic acid (OA) (57.30%), and linoleic acid (LA) (30.97%) were obtained in response to Zn and Ca nanofertilizers. The combined application of Zn and Ca nanofertilizers enhanced the SY of groundnut via increasing of PNP and 100-SW indices and improved the groundnut oil quality due to the increment of OA and LA under regional climatic conditions. Hence, Zn and Ca nanofertilizers showed superiority for the quantity and quality of groundnut yield and could be recommendable to enhance the quantitative and qualitative yield of groundnut under similar climatic conditions. [ABSTRACT FROM AUTHOR]

This study investigated the influence of different regimes of immersion batch frying of peanuts on its specific energy consumption. The investigation was conducted via simulation, where energy consumption was calculated using various heat power/peanut mass ratios. As the result of the applied optimization procedure within the examined domain and calculation data, it was estimated that a regime with 24 kW of heating power and 28.6 kg of peanuts gave the minimum specific energy consumption. Besides that, the resulting surface could serve as a basis for designing and operating the frying equipment in more favorable regimes in terms of energy efficiency. [ABSTRACT FROM AUTHOR]

BACKGROUND: Developing the stable and healthy emulsion-based food is in accord with the needs of people for health. In the present study, acidification at pH 3.0 of peanut polysaccharide (APPSI) was employed to regulate its conformation and further improve its advantages in preparing oil-in-water emulsion. RESULTS: The results indicated that acidification induced conversion of PPSI aggregates into linear chains. Increasing concentration promoted formation of cross-linked network structure shown in transmission electron microscopy images. Consequently, the viscosity, yield stress, storage modulus and flow activation energy significantly increased, further fabricating gel structure. Moreover, aggregation behavior suggested that more exposed proteins were involved in gel structure, thereby forming many hydrophobic cores as verified by fluorescence spectroscopy of pyrene. Afterwards, emulsion characteristics indicated that APPSI produced strong and thick steric hindrance around oil droplets and the coil-like interweaved chains locked the continuous phase, bringing strong elasticity and resistance to stress and creaming. Meanwhile, the lower fatty acid in APPSI-emulsion was released after simulated gastrointestinal digestion, mainly as a result of the high retention ratio of emulsion droplets. Furthermore, the elastic and viscous Lissajous curves suggested that the structure strength of APPSI-emulsion was similar to that of the salad dressing within the strain 53.22%. CONCLUSION: The conformation of PPSI after acidification at pH 3.0 was suitable for preparing the stable emulsion. The obtained emulsion could resist digestion and maintain a strong structure, comprising a cholesterol-free and low-fat salad dressing substitute. [ABSTRACT FROM AUTHOR]

BACKGROUND: The accumulation of microplastics in agricultural soil poses a threat to the sustainability of agriculture, impacting crop growth and soil health. Due to the geocarpy feature of peanut, geocarposphere soil environment is critical to pod development and its nutritional quality. While the effects of microplastics in the rhizosphere have been studied, their impact on peanut pod in the geocarposphere remains unknown. Biochar has emerged as a potential soil agent with the ability to remediate soil contamination. However, the mechanisms of biochar in mitigating the toxic effects of microplastics-contaminated geocarposphere soil on peanut pod development remain largely unexplored. RESULTS: We evaluated the peanut pod performance and microbiome when facing microplastics contamination and biochar amendment in geocarposphere soil. The results showed that microplastics present in geocarposphere soil could directly enter the peanut pod, cause pod developmental disorder and exert adverse effects on nutritional quality. Aberrant expression of key genes associated with amino acid metabolism, lipid synthesis, and auxin and ethylene signaling pathways were the underlying molecular mechanisms of microplastics-induced peanut pod developmental inhibition. However, these expression abnormalities could be reversed by biochar application. In addition, peanut geocarposphere microbiome results showed that biochar application could restore the diversity of microbial communities inhibited by microplastics contamination and promote the relative abundance of bacteria correlated with pathogen resistance and nitrogen cycle of geocarposphere soil, further promoting peanut pod development. CONCLUSION: This study demonstrated that biochar application is an effective strategy to mitigate the toxic effects of microplastics-contaminated geocarposphere soil on pod development and nutritional quality. [ABSTRACT FROM AUTHOR]

The toxicity of aluminum (Al) in acidic soil inhibits plant root development and reduces crop yields. In the plant response to Al toxicity, the initiation of programmed cell death (PCD) appears to be an important mechanism for the elimination of Al-damaged cells to ensure plant survival. In a previous study, the type I metacaspase AhMC1 was found to regulate the Al stress response and to be essential for Al-induced PCD. However, the mechanism by which AhMC1 is altered in the peanut response to Al stress remained unclear. Here, we show that a nuclear protein, mutator-like transposable element 9A (AhMULE9A), directly interacts with AhMC1 in vitro and in vivo. This interaction occurs in the nucleus in peanut and is weakened during Al stress. Furthermore, a conserved C2HC zinc finger domain of AhMULE9A (residues 735–751) was shown to be required for its interaction with AhMC1. Overexpression of AhMULE9A in Arabidopsis and peanut strongly inhibited root growth with a loss of root cell viability under Al treatment. Conversely, knock down of AhMULE9A in peanut significantly reduced Al uptake and Al inhibition of root growth, and alleviated the occurrence of typical hallmarks of Al-induced PCD. These findings provide novel insight into the regulation of Al-induced PCD. [ABSTRACT FROM AUTHOR]

The increasing number of IoT devices has led to more electronic waste production, which harms the environment and human health. Self-powered sensor systems are a solution, but they often use toxic materials. We propose using biocompatible peanut skin as the active material for a self-powered humidity sensor (PSP-SPHS) through integration with a peanut-skin-based triboelectric nanogenerator (PSP-TENG). The PSP-TENG was characterized electrically and showed promising results, including an open circuit voltage (162 V), short circuit current (0.2 µA), and instantaneous power (2.2 mW) at a loading resistance of 20 MΩ. Peanut skin is a great choice for the sensor due to its porous surface, large surface area, eco-friendliness, and affordability. PSP-TENG was further used as a power source for the PSP-humidity sensor. PSP-SPHS worked as a humidity-dependent resistor, whose resistance decreased with increasing relative humidity (%RH), which further resulted in decreasing voltage across the humidity sensor. This proposed PSP-SPHS exhibited a good sensitivity (0.8 V/RH%), fast response/recovery time (4/10 s), along with excellent stability and repeatability, making it a potential candidate for self-powered humidity sensor technology. [ABSTRACT FROM AUTHOR]

BACKGROUND: Peanut is a significant source of nutrition and a valuable oilseed crop. It is also a serious allergy source, which poses a threat to 1.1% of the population. This study aimed to screen lactic acid bacteria (LAB) with the capacity to alleviate peanut allergenicity and exhibit anti‐allergic properties. RESULT: The results show that LAB can make use of substances in peanuts to reduce the pH of peanut milk from 6.603 to 3.593–4.500 by acid production and that it can utilize the protein in peanuts to reduce the allergenic content (especially Ara h 1) and improve biological activity in peanut pulp. The content of Ara h 1 peanut‐sensitizing protein was reduced by 74.65% after fermentation. The protein extracted from fermented peanut pulp is more readily digestible by gastrointestinal juices. The inhibitory activity assay of hyaluronidase (an enzyme with strong correlation to allergy) increased from 46.65% to a maximum of 90.57% to reveal that LAB fermentation of peanut pulp exhibited a robust anti‐allergic response. CONCLUSION: The strains identified in this study exhibited the ability to mitigate peanut allergenicity partially and to possess potential anti‐allergic properties. Lactobacillus plantarum P1 and Lactobacillus salivarius C24 were identified as the most promising strains and were selected for further research. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

[Display omitted] • Heavy metals accumulate in biochar after contaminated peanut straw liquefaction. • Bioavailable fraction of heavy metals was converted to stable fraction in biochar. • Cd mobility was high in bio-oil, Zn and Cu were bound to unexchangeable fraction. • Leachability and phytoaccessibility of heavy metals were substantially reduced. • Heavy metal risk was reduced from medium and high risk to no and low risk. In this study, the speciation, leachability, phytoaccessibility, and environmental risks of heavy metals (Cd, Zn, and Cu) during liquefaction of contaminated peanut straw in ethanol at different temperatures (220, 260, 300, 340, and 380 °C) were comprehensively investigated. The results showed that elevated temperatures facilitated heavy metal accumulation in the biochar. The acid-soluble/exchangeable and reducible fraction percentages of heavy metals were substantially reduced in the biochar after liquefaction as the temperature increased, and the oxidizable fraction became the dominant heavy metal fraction, accounting for 44.14–78.67%. Furthermore, although an excessively high liquefaction temperature (380 °C) increased the residual fraction percentages of Zn and Cu, it was detrimental to Cd immobilization. The acid-soluble/exchangeable Cd in the contaminated peanut straw readily migrates to the bio-oil during liquefaction, with the highest concentration of 1.60 mg/kg at 260 °C liquefaction temperature, whereas Zn and Cu are predominantly bound to the unexchangeable fraction in the bio-oil. Liquefaction inhibited heavy metal leachability and phytoaccessibility in biochar, the lowest extraction rates of Cd, Zn, and Cu were 0.71%, 1.66% and 0.95% by diethylenetriamine pentaacetic acid, respectively. However, the leaching and extraction concentrations increased when the temperature was raised to 380 °C. Additionally, heavy metal risk was reduced from medium and high risk to no and low risk. In summary, liquefaction reduces heavy metal toxicity and the risks associated with contaminated peanut straw, and a temperature range of 300–340 °C for ethanol liquefaction can be considered optimal for stabilizing heavy metals. [ABSTRACT FROM AUTHOR]

Sugar Will Eventually be Exported Transporter (SWEET) proteins are highly conserved in various organisms and play crucial roles in sugar transport processes. However, SWEET proteins in peanuts, an essential leguminous crop worldwide, remain lacking in systematic characterization. Here, we identified 94 SWEET genes encoding the conservative MtN3/saliva domains in three peanut species, including 47 in Arachis hypogea, 23 in Arachis duranensis, and 24 in Arachis ipaensis. We observed significant variations in the exon-intron structure of these genes, while the motifs and domain structures remained highly conserved. Phylogenetic analysis enabled us to categorize the predicted 286 SWEET proteins from eleven species into seven distinct groups. Whole genome duplication/segment duplication and tandem duplication were the primary mechanisms contributing to the expansion of the total number of SWEET genes. In addition, an investigation of cis-elements in the potential promoter regions and expression profiles across 22 samples uncovered the diverse expression patterns of AhSWEET genes in peanuts. AhSWEET24, with the highest expression level in seeds from A. hypogaea Tifrunner, was observed to be localized on both the plasma membrane and endoplasmic reticulum membrane. Moreover, qRT-PCR results suggested that twelve seed-expressed AhSWEET genes were important in the regulation of seed development across four different peanut varieties. Together, our results provide a foundational basis for future investigations into the functions of SWEET genes in peanuts, especially in the process of seed development. [ABSTRACT FROM AUTHOR]

Anaphylaxis is a life-threatening reaction characterized by the acute onset of symptoms involving different organ systems and requiring immediate medical intervention. The incidence of fatal food anaphylaxis is 0.03 to 0.3 million/people/year. Most fatal food-induced anaphylaxis occurs in the second and third decades of life. The identified risk factors include the delayed use of epinephrine, the presence of asthma, the use of recreational drugs (alcohol, nicotine, cannabis, etc.), and an upright position. In the United Kingdom (UK) and Canada, the reported leading causal foods are peanuts and tree nuts. In Italy, milk seems to be the most common cause of fatal anaphylaxis in children < 18 years. Fatal food anaphylaxis in Italian children and adolescents almost always occurs outside and is characterized by cardiorespiratory arrest; auto-injectable adrenaline intramuscular was available in few cases. Mortality from food anaphylaxis, especially in children, is a very rare event with stable incidence, but its risk deeply impacts the quality of life of patients with food allergy and their families. Prevention of fatal food anaphylaxis must involve patients and their families, as well as the general public, public authorities, and patients' associations. [ABSTRACT FROM AUTHOR]

This study investigated the growth performance, nitrogen balance, and economics of production of Red Sokoto bucks (RSBs) fed Saccharomyces cerevisiaetreated, biodegradable groundnut haulms (SCGH) and dried cassava peels (DCP). Four dietary treatments were formulated including a control diet (T1; 0% SCGH:DCP) and DCP in combination with SCGH at the different proportions of 275:695 (T2), 375:595 (T3), and 475:495 (T4) g/kg of dry matter (DM). A combination of 5 g and 50 g of yeast as a probiotic and molasses, respectively, were added to 1 kg of GH after been dissolved in 1 L of water. Twelve healthy RSBs weighing 9.5 ± 0.40 kg of body weight (BW) and aged 7 to 8 months were exposed to four treatments. This was replicated three times in a completely randomized design (CRD). The experiment lasted for 90 days. The average final weight, average daily weight gain (gram/day), nitrogen intake, and nitrogen balance were significantly influenced by the inclusion of DCP and SCGH in the diets. There was no significant difference (p > 0.05) between the ADG of T1 (58.20 g/d) and the T4 (58.89 g/d) groups. Feed conversion ratio and feed-cost saving ($630.80) increased with the inclusion of DCP and SCGH in the goats' diets. In conclusion, T4 (SCGH: 475g, DCP: 495g) had a better chemical composition, ADG, nitrogen balance, and feed-cost saving; therefore, SCGH are recommended and are readily available agricultural wastes that can be fed to RSBs to improve performance and nitrogen utilization in a cost-effective way, especially during dry season. [ABSTRACT FROM AUTHOR]

Nothopassalora personata is one of the most economically severe pathogens of peanut in the United States. The fungus primarily relies on wind and rain for dispersal, which has been documented up to 10 m from an inoculum source. Spore traps have been used in a wide variety of pathosystems to study epidemiology, document detection, develop alert systems, and guide management programs. The objective of this study was to use spore traps and N. personata-specific qPCR primers to quantitatively evaluate dispersal of N. personata conidia at distances up to 70 m from an infected peanut field and to examine relationships between quantities captured and weather variables. Impaction spore samplers were placed at 4, 10, 30, 50, and 70 m from peanut fields at the Edisto Research and Education Center (six fields) and commercial peanut fields in Barnwell and Bamberg counties (one field each) from 2020 to 2022. Following initial detection, samples were collected at a 48-, 48-, 72-h interval until harvest. N. personata conidia were detected at all locations and distances, documenting dispersal up to 70 m from an inoculum source. This result is a reminder that volunteer management is crucial when rotating peanut in nearby fields. A model for predicting log spore quantities was developed using temperature and humidity variables. Temperature variables associated with observed sampling periods had a negative correlation with N. personata quantities, whereas parameters of relative humidity and mean windspeed were positively correlated. [ABSTRACT FROM AUTHOR]

A-genome Arachis species (AA; 2n = 2x = 20) are commonly used as secondary germplasm sources in cultivated peanut breeding, Arachis hypogaea L. (AABB; 2n = 4x = 40), for the introgression of various biotic and abiotic stress resistance genes. Genome doubling is critical to overcoming the hybridization barrier of infertility that arises from ploidy-level differences between wild germplasm and cultivated peanuts. To develop improved genome doubling methods, four trials of various concentrations of the mitotic inhibitor treatments colchicine, oryzalin, and trifluralin were tested on the seedlings and seeds of three A-genome species, A. cardenasii, A. correntina, and A. diogoi. A total of 494 seeds/seedlings were treated in the present four trials, with trials 1 to 3 including different concentrations of the three chemical treatments on seedlings, and trial 4 focusing on the treatment period of 5 mM colchicine solution treatment of seeds. A small number of tetraploids were produced from the colchicine and oryzalin gel treatments of seedlings, but all these tetraploid seedlings reverted to diploid or mixoploid states within six months of treatment. In contrast, the 6-h colchicine solution treatment of seeds showed the highest tetraploid conversion rate (6–13% of total treated seeds or 25–40% of surviving seedlings), and the tetraploid plants were repeatedly tested as stable tetraploids. In addition, visibly and statistically larger leaves and flowers were produced by the tetraploid versions of these three species compared to their diploid versions. As a result, stable tetraploid plants of each A-genome species were produced, and a 5 mM colchicine seed treatment is recommended for A-genome and related wild Arachis species genome doubling. [ABSTRACT FROM AUTHOR]

Phenylalanine ammonia-lyase (PAL) is an essential enzyme in the phenylpropanoid pathway, in which numerous aromatic intermediate metabolites play significant roles in plant growth, adaptation, and disease resistance. Cultivated peanuts are highly susceptible to Aspergillus flavus L. infection. Although PAL genes have been characterized in various major crops, no systematic studies have been conducted in cultivated peanuts, especially in response to A. flavus infection. In the present study, a systematic genome-wide analysis was conducted to identify PAL genes in the Arachis hypogaea L. genome. Ten AhPAL genes were distributed unevenly on nine A. hypogaea chromosomes. Based on phylogenetic analysis, the AhPAL proteins were classified into three groups. Structural and conserved motif analysis of PAL genes in A. hypogaea revealed that all peanut PAL genes contained one intron and ten motifs in the conserved domains. Furthermore, synteny analysis indicated that the ten AhPAL genes could be categorized into five pairs and that each AhPAL gene had a homologous gene in the wild-type peanut. Cis-element analysis revealed that the promoter region of the AhPAL gene family was rich in stress- and hormone-related elements. Expression analysis indicated that genes from Group I (AhPAL1 and AhPAL2), which had large number of ABRE, WUN, and ARE elements in the promoter, played a strong role in response to A. flavus stress. [ABSTRACT FROM AUTHOR]

Background: Peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) is a major constraint for groundnut production in Andhra Pradesh (A.P.). However, studies on prevalence and spread of the disease confined to only few districts of A.P. with this background current study focused on incidence and spread of the disease in entire state of A.P. Further an isolate of TSV occurring in A.P. characterized on the basis of genetic features by comparing with other TSV isolates originated from different hosts and locations from world. Methods: Roving survey was conducted during kharif 2017-18 in groundnut growing districts of Andhra Pradesh (A.P.) for peanut stem necrosis disease incidence. Groundnut plants showing PSND symptoms were collected and tested with direct antigen coating enzyme linked immunosorbent assay (DAC-ELISA). Groundnut samples found positive by ELISA once again tested by reverse transcription polymerase chain reaction (RT-PCR). The representative TSV-GN-INDVP groundnut isolate from Prakasham district was maintained on cowpea seedlings by standard sap inoculation method in glasshouse for further molecular characterization. The Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny. Result: Thirty-eight (52.7%) of seventy-two groundnut samples collected from different locations in A.P were given positive reaction to TSV by DAC-ELISA. For the first time, PSND incidence observed in coastal districts (Krishna, Guntur, Sri Pottisriramulu Nellore, Prakasham) of A.P. Maximum PSND incidence recorded from Bathalapalli (22.2%) and the minimum incidence in Mulakalacheruvu (4.1%). The coat protein (CP) gene of TSV-GN-INDVP groundnut isolate was amplified by RT-PCR and it shared maximum per cent nucleotide identity (97.51-98.62%) with TSV isolates from groundnut and other different crops reported in India. All Indian isolates cluster together irrespective of crop and location based on the phylogenetic analysis. [ABSTRACT FROM AUTHOR]

Background: Early leaf spot caused by Cercospora arachidicola Hori. is most destructive disease in all the groundnut growing areas of Rajasthan, under severe infestation it cause 30-50% losses in pod yield. Recently, Central Insecticide Board (CIB), Faridabade banned 27 pesticides including some important fungicides which are extensively used in plant disease management. Therefore, in the present investigation, our main emphasis was to find out some new fungicides for management of early leaf spot disease. Methods: Ten fungicides (hexaconazole 5% EC, difenconazole 25% EC, propiconazole 25% EC, tebuconazole 25.9% EC, trifloxystrobin 25% + tebuconazole 50% WG, mancozeb 50% WP, chlorothalonil 75% WP, carbendazim 50% WP, carbendazim 12% + mancozeb 63% WP and captan 70% + hexaconazole 5% WP) were evaluated against early leaf spot pathogen (C. arachidicola) both in lab as well as in field condition. The experiment was conducted at ARS, SKRAU, Bikaner during Kharif-2019 on most popular cv. HNG-69 in RBD design with the application of foliar spray of ten different fungicides at different concentrations against early leaf spot disease and compared with an untreated control. Result: Among all the fungicides used in the present investigation, Tebuconazole 25.9% EC was found most effective in inhibiting the mycelial growth of the pathogen followed by trifloxystrobin 25% + tebuconazole 50% WG. Under field condition, it gave maximum (70.73%) disease control with highest pod yield (31.5 q/ha) and net return (Rs 57,500/ha) when applied as foliar spray at 0.1% concentration followed by trifloxystrobin 25% + tebuconazole 50% WG at 0.2%. These treatments can provide an effective and economical management of early leaf spot disease for groundnut cultivators. [ABSTRACT FROM AUTHOR]

Background: Peanut is a widely cultivated food crop of the legume family and is a major source of vegetable oil and protein in the global agricultural economy. However, the productivity of peanuts is severely affected by abiotic stress, particularly drought. Therefore, it is necessary to identify genes related to abiotic stress tolerance and analyze genetic diversity by SSR markers related to drought tolerance in peanut varieties. Methods: PCR amplification was used for the isolation of the DREB2C gene. The DREB2C gene sequence was analyzed using bioinformatic tools to identify functional domains, 3D modeling structures and other important characteristics. RT-qPCR was performed to evaluate the expression level of the DREB2C gene in the different tissues of peanut cultivars. A phylogenetic tree was constructed based on SSR markers linked to drought tolerance to assess the genetic diversity among peanut cultivars. Result: The present study identified the DREB2C gene in peanut cultivar L14. The gene encodes a polypeptide chain of 492 amino acids lacking transmembrane domains and signal peptides and with a conserved domain (AP2/ERF) and DNA binding site. The 3D protein structure was predicted with high confidence using various tools. The gene expression was investigated in different tissues and at different growth and developmental stages, as well as in various cultivars. Based on SSR markers linked to drought tolerance, the study revealed that peanut cultivars in Vietnam exhibit a medium level of diversity. It may be suggested that crossbreeding between different groups may increase variability among crops, allowing the generation of dominant varieties with improved drought tolerance. [ABSTRACT FROM AUTHOR]

Background: Heavy metal toxicity affects plant growth and alters physiological processes. Soils in many areas are often contaminated by cadmium and zinc which show varied response on plants by their interactive effects. The experiment was done to study the effect of cadmium and zinc as sole presence and in combination in groundnut seedlings. Methods: The laboratory experiment was conducted on groundnut cultivar TG 51 in sand culture using modified Hoagland solution. After initial screening, three concentrations of cadmium (Cd 100, Cd 300 and Cd 500 μM) and two concentrations of zinc (Zn 50 and Zn 150 μM) were selected for studying their effects individually and in combination on physiological and biochemical parameters. Result: The reduction in root length increased over control as the concentration of cadmium in the medium increased. Cadmium or zinc alone led to a decrease in chlorophyll a, b and relative water content of the leaf. Zinc supplement at 150 µM not only mitigated the negative effect of Cd 100 µM and 300 µM, but also increased the chlorophyll content above control level. Zinc supplement not only increased the protein content over the control but also mitigated to some extent the adverse effects of cadmium in protein content when applied in combination. Under both cadmium and zinc treatment, the inhibition of nitrate reductase (NR) activity over unstressed control was found. Different treatment combinations, however, reduced the negative effects of cadmium, although zinc could not completely override such damage, change the level of toxicity. Treatment with Cd 100 µM and 300 µM induced an increase in phenol content over the control, while higher concentration (500 µM) of the metal led to a decrease in this potent antioxidant compound. Presence of Zinc in the growing medium significantly enhanced the accumulation of phenolic compounds highlighting its protective role against oxidative damage. [ABSTRACT FROM AUTHOR]

The research aimed to study the production situation and the productive and economic indicators of the maize crop to estimate and analyze farmers’ responses to farm prices and some other economic variables during the period (2000-2021). This was done by identifying the factors that most influence the area cultivated with the maize crop through estimating the response functions of the supply of the maize crop in comparison with the competing summer crops. Also, the degree of farmers’ response and the time period required to achieve this response was studied. To achieve the objectives of the study, descriptive and quantitative statistical analysis methods were used, through the Nerlov model to estimate the response functions of the maize crop area in the linear form. The study relied on published and unpublished secondary data from the Ministry of Agriculture and Land Reclamation during the period (2000-2021). The study dealt with the development of the area cultivated with maize during the period (2001-2021). The average area cultivated with maize in 2001 was about 1773.5 thousand fed, and in 2021 it reached about 2246.9 thousand fed, meaning that it increased by about 27%. The average productivity per fed during the year 2001 was about 3.44 tons/ fed, while it decreased in 2021 to reach about 3.32 tons/ fed. The national consumption of corn in Egypt in 2001 reached about 11.23 million tons, and it increased to reach about 17.086 million tons in 2021, with average increase 54%. The average per capita share of the maize crop in 2001 was about 54.3 kg/year, while the year 2021 reached about 43.7 kg/year, meaning that it decreased in 2021 than 2001 by about 15%. The study also found a decline in the economic efficiency of the maize crop for the period (2001-2021), as the average amounted to the average profitability of the invested pound, the relative profitability, and the producer incentive are about 0.56, 0.82 and 10.59, with a decreasing annual growth rate of about 4.1%, 4.5% and 2.5%, respectively. By estimating the maize supply response functions according to the farm price, the cost of producing a ton, and the ton net return of the same crop during the period from (2001- 2021) is directly proportional to the area slowing down a year. By estimating the response functions of the maize crop using one variable with the area, slowing down a year, it turns out that the response of the cultivated area of maize during the study period (2001- 2021) was directly proportional to the cost of a ton of soybeans, the cost of a ton of sunflowers, the cost of a ton of rice, the cost of a ton of blossom cotton, the cost of a ton of summer tomatoes, the cost of a ton of peanuts, the ratio of the net yield of corn/sunflowers, the ratio of net yield of corn/ rice. [ABSTRACT FROM AUTHOR]

Aim This study aimed to isolate, endophytic Streptomyces sp. MSARE05 isolated from root of a peanut (Arachis hypogaea) inhibits the growth of other bacteria. The research focused on characterizing the strain and the antimicrobial compound. Methods and Results The surface-sterilized peanut roots were used to isolate the endophytic bacterium Streptomyces sp. MSARE05. A small-scale fermentation was done to get the antimicrobial compound SM05 produced in highest amount in ISP-2 medium (pH 7) for 7 days at 30°C in shaking (180 rpm) condition. Extraction, purification, and chemical analysis of the antibacterial component revealed a novel class of antibiotics with a 485.54 Dalton molecular weight. The MIC was 0.4–0.8 µg ml−1 against the tested pathogens. It also inhibits multidrug-resistant (MDR) pathogens and Mycobacterium with 0.8–3.2 µg ml−1 MIC. SM05 was found to disrupt cell membrane of target pathogen as evident by significant leakage of intracellular proteins and nucleic acids. It showed synergistic activity with ampicillin, chloramphenicol, streptomycin, and kanamycin. Conclusions The new-class antimicrobial SM05 consisting naphthalene core moiety was effective against drug-resistant pathogens but non-cytotoxic to human cells. This study underscores the significance of endophytic Streptomyces as a source of innovative antibiotics, contributing to the ongoing efforts to combat antibiotic resistance. [ABSTRACT FROM AUTHOR]

Drought stress is an important challenge to global food security and agricultural output, and dramatic and rapid climate change has made the problem worse, causing unexpected impacts on the growth, development, and yield of different plants. Understanding the biochemical, ecological, and physiological reactions to these pressures is essential for improved management. Carbon materials' impacts on plants subjected to different stresses are still poorly studied. Thus, this study was carried out investigate the feasibility of applying carbon nanotubes (CNTs) (0, 20, and 40 mg/L) as a foliar treatment for mitigating the effect of water stress (100%, 75%, and 50% irrigation water, IW) on peanut plants growing in sandy soil through assessments of growth and productivity and some physiological and biochemical measurements. Exposure of peanuts to decreased irrigation water led to significant decreases in growth, yield, photosynthetic pigments, indole acetic acid (IAA), and some nutritional components in peanut seeds, but increased levels of osmolytes such as total soluble carbohydrates (TSS) and proline, in addition to free amino acids and phenolics. However, foliar spraying with CNTs could ameliorate the impacts of decreased irrigation water on growth and production via enhancing the studied physiological parameters, such as photosynthetic pigments, IAA, osmolytes, and phenolics. Furthermore, the application of carbon nanotubes improved the nutrient contents, as expressed by the oil yield, protein yield, total carbohydrates, antioxidant activities (DPPH), B-carotene, lycopene, and flavonoids in peanut seeds, either under normal or water stress conditions. The higher level of CNTs (40 mg/L) was more effective than the lower one (20 mg/L) at increasing the above-mentioned parameters. In conclusion, foliar treatment with carbon nanotubes has the ability to enhance peanut drought tolerance and increase its growth and productivity under sandy soil conditions. [ABSTRACT FROM AUTHOR]

Peanut is a leguminous crop with an indeterminate growth habit that will continuously flower during the entire reproductive development stage. Flowering redundancy adversely affects the yield and quality of peanut. In this study, eight flowering and five yield-related traits were collected and comprehensively evaluated from a diverse germplasm panel consisting of 232 peanut accessions, aiming to provide a theoretical basis for improving the flowering habit and yield for future peanut breeding efforts. As a result, large phenotypic diversity was observed in 13 traits. Most of the traits suggested high heritability, except high effective flowering duration (HEFD), days to 100% flower cessation (DTC100), and yield per plant (YPP). Days to 90% flower cessation (DTC90), days to DTC100, flowering duration (FD), and low effective flowering duration (LEFD) showed significant negative correlations with 100-seed weight, 100-pod weight, YPP, average weight per pod, and shelling percentage. Principal component analysis (PCA) suggested that flowering redundancy traits as well as yield-related traits contributed more to the first three PCs when compared to other traits. This study addresses the lack of peanut flowering phenotypic and genetic diversity and lays the foundation for in-depth research on redundancy-related genes in peanut flowering. [ABSTRACT FROM AUTHOR]

Much evidence supports that the early introduction of allergenic foods in weaning is useful to prevent food allergies later in life. Real life is often different, with factors related to mothers and infants. Our study aimed to deepen the timing of introducing the foods responsible for most allergic reactions during the weaning and why parents delay their introduction. 110 mothers participated in the study, compiling a questionnaire. Exclusive breastfeeding was associated with a delayed introduction of allergenic foods at 4 months (r = 0.433, p < 0.01) and 1 year (r = 0.486, p < 0.01). Large-for-gestational age at birth was inversely associated with a delayed introduction of allergenic foods (r=-0.204, p < 0.05). This study demonstrates that introducing many allergens is delayed during the weaning. Parents with infants fed with exclusive breastfeeding could need more information about the correct time of introduction of potential allergens in the weaning. [ABSTRACT FROM AUTHOR]

Wild relatives comprise a diverse genetic source from which to introduce beneficial alleles for peanut breeding. However, when using wild species for breeding, unadapted agronomic characteristics are often co‐inherited along with favorable traits. Previously, an advanced backcross population was developed from a cross between Arachis hypogaea and the wild‐derived induced allotetraploid (Arachis batizocoi × Arachis stenosperma)4x to incorporate nematode resistance genes into elite peanut. We observed additional wild alleles unintentionally inherited throughout the genome, possibly causing variation in several pod and seed characteristics. To identify the wild introgressions controlling the variation in pod constriction (PC), a critical trait for peanut's acceptability and marketability, we performed an association analysis using 419 SNPs and phenotypic data of 72 BC3F2/3 families. An introgression on the top of chromosome B08 (pod constriction‐quantitative trait locus [PC‐QTL], 3.7 Mbp) derived from A. batizocoi was found to be controlling 47.3% of the PC variation. To confirm the PC‐QTL, six BC3F4 families harboring the wild loci in a homozygous or heterozygous state were selected and used for segregation analysis. All progenies from the backcross families carrying the homozygous allele had severely constricted pods, whereas progenies from families with the heterozygous allele were segregated for PC. Finally, analyses using end‐point genotyping with four linked SNP assays confirmed the association of the PC‐QTL with PC. The present work studied PC and developed DNA markers to assist the selection against constricted pods while retaining the new and beneficial wild‐derived genes. Core Ideas: In peanut, pod and seed traits are important for yield and quality.When incorporating beneficial traits from wild germplasm, unadapted wild pod and seed traits are also inherited.A major pod constriction quantitative trait locus was discovered in a backcross population harboring wild‐derived segments.Molecular markers developed and validated can be used for selection against wild‐type deep pod constriction. [ABSTRACT FROM AUTHOR]

The present study used gas chromatography–mass spectrometry (GC–MS) and gas chromatography–ion mobility spectrometry (GC–IMS) to separate and identify the characteristic volatile flavor substances in 30 roasted peanut samples. GC–MS identified 59 volatile compounds, and GC–IMS detected 61 volatile flavor substances. The 30 peanut varieties were then divided into four groups on the basis of their volatile flavor substances using principal component analysis (PCA), and a fingerprint profile of the varieties' volatile characteristics was established from information peaks identified in the spectra. Descriptive sensory analysis (DSA) was performed to distinguish differences in flavor attributes between roasted peanut varieties. Partial least squares regression (PLSR) was performed with the volatile flavor content of roasted peanuts as the independent variable and the flavor attribute score as the dependent variable. These findings provide a basis for predicting the appeal of roasted peanuts based on their composition and demonstrate a potential avenue for improving food flavor quality. [ABSTRACT FROM AUTHOR]

Summary: Cereal‐based diets supplemented with legumes have proven to be an effective approach to enhancing nutritional value. This study evaluated the consumer acceptability and metabolite profile of fermented sorghum mahewu improved with different proportions (20% and 30%) of Bambara groundnut (BGN). Consumer acceptability, metabolite profile analysis using gas chromatography high‐resolution time‐of‐flight mass spectrometry (GC‐HRTOF‐MS), storage stability and microbial quality of mahewu were determined. Sorghum (100SORG) mahewu without BGN was the most preferred by the consumer panellists followed by 80SORG:20BGN. Metabolites identified in all three mahewu products included esters (21%), alcohols (17%), alkanes (12%), acids and derivatives (9%). Overall, 70SORG:30BGN had an improved metabolite profile but least preferred by consumer. The sorghum‐BGN mahewu maintained their pH, while increases in consistency and TTA were observed with storage time and BGN supplementation. Inclusion of BGN in sorghum mahewu did not alter microbial quality; however, more awareness is required to increase its acceptance among consumers. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the feasibility of identifying the geographical origin of peanuts by combining Raman spectroscopy with chemometrics. A total of 161 peanut samples were collected from Jilin, Jiangsu, and Shandong provinces in China, and their Raman spectra were collected. One-way analysis of variance (ANOVA) was used to analyze the difference in characteristic Raman spectra of peanuts from these locations. Raman spectroscopy combined with principal component analysis (PCA), k-nearest neighbor (k-NN), stepwise linear discriminant analysis (SLDA), and support vector machines (SVM) were used to classify the peanuts by province and Jilin Province city. One-way ANOVA indicated that the peak intensities at 2900, 1660, 1440, 1077, and 848 cm−1 had significant differences. The peaks at 2900, 1660, 1440, 1300, and 1077 cm−1 had significant differences in the Jilin Province city. The correct identification rates were highest for k-NN. This study demonstrates the identification of the origin of peanuts by Raman spectroscopy with chemometrics and may provide technical support for the traceability of other agricultural products. [ABSTRACT FROM AUTHOR]

Microbial fertilizer (MF) provides nutrition to the soil while also improving its structure. Moreover, plants are grown healthy with the help of MF, including beneficial microorganisms and fertilizer. In this study, it was aimed to determine the effects of MFs applied in different growth stages on yield and yield components of peanut (Arachis hypogaea L.). The study was conducted in Osmaniye, Eastern Mediterranean, Türkiye, in 2019 and 2020 according to a split-plot design with three replications. Growth stages of peanut (beginning bloom, full bloom, beginning bloom + full bloom) were placed in main plots and MF doses (0, 50, 100, and 150 cc ha−1) were placed in sub-plots. Consequently, the highest pod yield was observed in 100 and 150 cc ha−1 with the values of 603.67 kg da−1 in 2019 and 590.09 kg da−1 in 2020. For growth stages, full bloom treatments (605.34 kg da−1 in 2019 and 610.81 kg da−1 in 2020) came to the forefront in both years. The treatments of the beginning bloom (23.56% in 2019 and 23.77% in 2020) and 100 cc ha−1 (23.64% in 2019 and 23.88% in 2020) reached the maximum protein content in the studied years. Finally, the present study indicated that MFs had positive effects on yield components of peanut, especially with treatment150 cc ha−1 which increased pod yield and protein content at full bloom stage. [ABSTRACT FROM AUTHOR]

This research was conducted to determine the effect of the percentage of non-dairy cream and Moringa leaves filtrate on the characteristics of peanut ice cream. This research used a Completely Randomized Design (RAL) method with two factors i.e the percentage of non-dairy cream (10, 20%, 30%, and 40%) and the percentage of Moringa leaves filtrate (0%, 10%, 20%, and 30%) with three repetitions. The results showed that the percentage of non-dairy cream had a highly significant effect on fat and water content, total dissolved solids, overrun, melting time, and hedonic value of texture. The percentage of Moringa leaves filtrate had a highly significant effect on protein, fat, and water content vitamin C content, total dissolved solids, overrun, melting time, and hedonic value of taste. Interaction between the percentage of non-dairy cream and moringa leaves filtrate had a highly significant effect on fat content, total soluble solids, and overrun. Antioxidant analysis was conducted on the best treatment with a percentage of 40% non-dairy cream and 30% Moringa leaves filtrate with IC50 of 146.6368 ppm.

Background: Nuts and seeds have been part of diets in most of the world for millenniums, and they have also been consumed in the Nordic and Baltic countries for centuries. Consumption of nuts and seeds is linked with various health outcomes. Therefore, when updating the Nordic Nutrition Recommendations (NNR), summarizing the best evidence on key health outcomes from the consumption of nuts and seeds is essential. Objectives: This study aims to evaluate the updated evidence on the consumption of nuts and seeds and health outcomes regarded relevant for the Nordic and Baltic countries, as well as their dose-response relationship presented in updated systematic reviews and meta-analyses. Method: The scoping review is built on a de novo systematic review and an umbrella review published in 2022 on the consumption of nuts and seeds and its various health outcomes, including cardiovascular disease and diabetes. Results: Intake of nuts and seeds is associated with a lower risk of cardiovascular diseases, with evidence assessed as probable. This conclusion is mirrored by evidence from trials on biomarkers for chronic diseases. An intake of a serving of nuts of 28–30 g/day compared to not eating nuts is estimated to translate into approximately 20% relative reduction in the risks of cardiovascular disease and premature deaths. For cancers, consumption of a serving of nuts is inversely associated with cancer mortality. However, for type 2 diabetes, there are mixed and inconclusive results. Additionally, there are inverse associations between nut consumption and respiratory and infectious disease mortality. Allergies for nuts are seen among 1–2% of the population. Conclusion: Overall, the current evidence supports dietary recommendations to increase nut consumption to a serving of nuts and seeds per day for people without allergies to these foods.

Simple synthesis of peanut shell-like MoCoFe-HO@CoMo-LDH for efficient alkaline oxygen evolution reaction. [Display omitted] • Novel electrocatalysts with core–shell structure formed through self-assembly strategies. • MoCoFe-HO@CoMo-LDH has a strong ability to accelerate electron transfer and promote oxygen evolution kinetics. • MoCoFe-HO@CoMo-LDH hybrid materials exhibit high electrocatalytic performance in OER. Due to the depletion of fossil energy on earth, it is crucial to develop resource rich and efficient non-precious metal electrocatalysts for oxygen evolution reaction (OER). Herein, we synthesized an efficient and economical electrocatalyst using a simple self-assembly strategy. Firstly, rod-shaped MIL-88A was synthesized by hydrothermal method. Then, the surface of MIL-88A was functionalized and encapsulated in zeolitic imidazolate framework-67 (ZIF-67) by hydrothermal method. The combination of MIL-88A and ZIF-67 resulted in a slight ion-exchange reaction between Co2+ and the surface of MIL-88A to generate CoFe-LDH@ZIF-67 core–shell structure. Afterwards, in the presence of Mo6+, ZIF-67 was converted into CoMo-nanocages through ion-exchange reactions, forming a core–shell structure of MoCoFe hydr (oxy) oxide@CoMo-LDH (MoCoFe-HO@CoMo-LDH). Due to the advantages of core–shell structure and composition, this material exhibits excellent OER characteristics, with a small Tafel slope (45.11 mV dec-1) and low overpotential (324 mV) at 10 mA cm−2. It exhibits good stability in alkaline media. This research work provides a novel approach for the development of efficient and economical non-precious metal electrocatalysts. [ABSTRACT FROM AUTHOR]

In the nut processing industry, such as peanuts, the skin peeling process is an important stage to obtain a quality final product. Manual peeling can be a tiring and time-consuming job, so automating the peeling process using a machine is an efficient solution. The aim of designing a peanut shelling machine is to make it easier for peanut farmers to make the production process more efficient. The design stage of the peanut skin peeling machine design includes analysis of data requirements and obtaining a design that suits the farmer's capacity, design design using Autodesk Inventor Professional Software and design analysis of the peanut skin peeling machine design. The results of the analysis of the peanut shelling machine frame experienced a displacement of 0.00352 – 0.01758 and a safety factor of 15 sf, with stress of -0.886 Mpa to 0.437 Mpa which was experienced at a point below the yield stress of 1.088 Mpa so that the chassis construction was declared safe. to the static load received.

India is fulfilling the consumption requirement of its pulses and oilseeds largely through importing. Andhra Pradesh is a leading state in the country, significantly contributes to the production of these crops. Low yield of pulses and groundnuts in India should be addressed through adoption of proven technological interventions along with enhancing farmers knowledge. The present study aimed to determine the differences in knowledge of Sustainable Agricultural Practices (SAPs) and adoption of improved agricultural practices (IAPs) among farmers at the baseline and endline phase of the study. The association of possible factors such as age, gender, education, farm experience, mass media, social participation, risk orientation, innovativeness with knowledge and adoption of Sustainable agricultural practices was evaluated. The study also examined the result of the technological intervention on crop yield at pre and post intervention. The study included 240 farmers with poor pulse and groundnut yield from villages of Andhra Pradesh with inadequate technological developments. At biotech intervention phase, farmers received training, field demonstration etc. The results revealed that at endline, 80% of farmers had knowledge of SAPs (compared to 48% at baseline) and the adoption rate of IAPs was 50% (compared to 3% at baseline). Factors such as mass media, social participation, risk orientation showed significant reduced risk on farmers with high knowledge of SAPs and with complete adoption of IAPs. The average yield per hectare of pulses during baseline was found to be 403.5 kg/ha ± 128.4 while during endline it was 601.25 kg/ha ± 206.8 (p-value = 0.001). The average yield per hectare of groundnut during baseline was found to be 983.75 kg/ha ± 444.9 and during endline it was 1216.78 kg/ha ± 473.9 (p-value = 0.000). Innovative technological interventions and capacity building of farmers increased yield of crops in Andhra Pradesh. [ABSTRACT FROM AUTHOR]

Background: Calmodulins (CaMs)/CaM-like proteins (CMLs) are crucial Ca2+-binding sensors that can decode and transduce Ca2+ signals during plant development and in response to various stimuli. The CaM/CML gene family has been characterized in many plant species, but this family has not yet been characterized and analyzed in peanut, especially for its functions in response to Ralstonia solanacearum. In this study, we performed a genome-wide analysis to analyze the CaM/CML genes and their functions in resistance to R. solanacearum. Results: Here, 67, 72, and 214 CaM/CML genes were identified from Arachis duranensis, Arachis ipaensis, and Arachis hypogaea, respectively. The genes were divided into nine subgroups (Groups I-IX) with relatively conserved exon‒intron structures and motif compositions. Gene duplication, which included whole-genome duplication, tandem repeats, scattered repeats, and unconnected repeats, produced approximately 81 pairs of homologous genes in the AhCaM/CML gene family. Allopolyploidization was the main reason for the greater number of AhCaM/CML members. The nonsynonymous (Ka) versus synonymous (Ks) substitution rates (less than 1.0) suggested that all homologous pairs underwent intensive purifying selection pressure during evolution. AhCML69 was constitutively expressed in different tissues of peanut plants and was involved in the response to R. solanacearum infection. The AhCML69 protein was localized in the cytoplasm and nucleus. Transient overexpression of AhCML69 in tobacco leaves increased resistance to R. solanacearum infection and induced the expression of defense-related genes, suggesting that AhCML69 is a positive regulator of disease resistance. Conclusions: This study provides the first comprehensive analysis of the AhCaM/CML gene family and potential genetic resources for the molecular design and breeding of peanut bacterial wilt resistance. [ABSTRACT FROM AUTHOR]

The plant growth-promoting rhizobacterium Tsukamurella tyrosinosolvens P9 can improve peanut growth. In this study, a co-culture system of strain P9 and peanut was established to analyze the transcriptome of peanut roots interacting with P9 for 24 and 72 h. During the early stage of co-culturing, genes related to mitogen-activated protein kinase (MAPK) and Ca2+ signal transduction, ethylene synthesis, and cell wall pectin degradation were induced, and the up-regulation of phenylpropanoid derivative, flavonoid, and isoflavone synthesis enhanced the defense response of peanut. The enhanced expression of genes associated with photosynthesis and carbon fixation, circadian rhythm regulation, indoleacetic acid (IAA) synthesis, and cytokinin decomposition promoted root growth and development. At the late stage of co-culturing, ethylene synthesis was reduced, whereas Ca2+ signal transduction, isoquinoline alkaloid synthesis, and ascorbate and aldarate metabolism were up-regulated, thereby maintaining root ROS homeostasis. Sugar decomposition and oxidative phosphorylation and nitrogen and fatty acid metabolism were induced, and peanut growth was significantly promoted. Finally, the gene expression of seedlings inoculated with strain P9 exhibited temporal differences. The results of our study, which explored transcriptional alterations of peanut root during interacting with P9, provide a basis for elucidating the growth-promoting mechanism of this bacterial strain in peanut. [ABSTRACT FROM AUTHOR]

Anisotropic colloidal particles can directionally self‐assemble, but the resulting assemblies cannot reconfigure unless the surface properties of colloidal particles change. Although external fields with adjustable inputs can guide the reconstruction of active structures after stimulating the dynamic assembly of active colloidal motors, the interaction between colloidal motors is unclear. Here, the study reports a bioinspired communication and directed assembly of peanut‐shaped colloidal motors with the emergence of ordered ribbon structures triggered by self‐generated chemical fields. The colloidal motors photocatalytically decompose the fuel to create the chemical concentration gradient fields and exert long‐range attractions on the colloidal scale, which enable colloidal motors to communicate by sensing each other, synchronizing orientation, and assembling side‐by‐side after the collision between their local flow fields. The inter‐individual communication occurs at a critical distance of ≈4.4 µm, resulting from the interplay between hydrodynamic effects, long‐ranged phoretic attraction, and short‐ranged magnetic interaction. The linear ribbons are still active owing to the symmetry breaking of the local fluid field and can be switched between rigid and flexible states according to light conditions to simulate the polymer conformation. The complexity of colloidal motors can be combined with external manipulations to manufacture active soft materials with precise and adaptive functions. [ABSTRACT FROM AUTHOR]